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Fig. 1 | BMC Biology

Fig. 1

From: Hybrid de novo genome assembly and centromere characterization of the gray mouse lemur (Microcebus murinus)

Fig. 1

Flowchart of hybrid assembly procedure. The initial assembly was generated using Illumina data and AllPaths-LG, followed by refined scaffolding using Atlas-Link and gap filling using Atlas-GapFill. Further gap filling with PacBio data and PBJelly followed, generating Mmur 2.0. The Mmur 2.0 assembly was super-scaffolded in an iterative method using BNG optical map data to identify conflicts, break and join scaffolds, and Lachesis and Hi-C proximity ligation data to further super-scaffold. The PBJelly method was used a second time to fill gaps in the final super-scaffolds followed by Pilon error correction, creating the Mmur 3.0 assembly (* indicates that the same PacBio data was used for the second PBJelly analysis)

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BMC Biology

ISSN: 1741-7007

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