Fig. 4
From: Sequence-based prediction of permissive stretches for internal protein tagging and knockdown
Stabilisation of nucleotide pool in CFX using conditional protein knockouts. Time course of nucleotide inter-conversion in CFX with or without pre-treatment by TEV protease a Adk: ADP was added to a CFX prepared from strain EcAdk76.3. b AtpA and c AtpD: ATP was added to a CFX prepared from strains EcAtpA and EcAtpD. Nucleotide concentrations were quantified at indicated time points by HPLC in triplicate ± SD. 95% confidence intervals indicated the accuracy of the fits. d Specific half-life times (min) with 95% confidence bounds (min) of ATP or ADP before and after knocking out enzymatic activity by TEV protease cleavage