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Fig. 2 | BMC Biology

Fig. 2

From: A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53

Fig. 2

Nutlin inhibits the interaction of p53 (1–52) with Mdm2 in ABC3Δ cells. a Overnight cultures of wild-type or ABC3Δ cells containing plasmids encoding either Gal4 AD-Csm1/Gal4 BD-Dsn1 (used as a negative control), Gal4 AD-p53/Gal4 BD-Mdm2, or Gal4 AD-p53 (1–52)/Gal4 BD-Mdm2 in non-selective medium were washed in water and inoculated at OD600 = 0.2 into selective medium containing DMSO or nutlin at the indicated concentrations in duplicate. For each strain, growth as measured by average OD600 of duplicate cultures is plotted against time. Ends of the vertical bar indicate the OD600 values of the duplicate cultures. b Overnight cultures of ABC3Δ cells containing plasmids encoding either Gal4 AD-Csm1/Gal4 BD-Dsn1, Gal4 AD-p53 (1–52)/Gal4 BD-Mdm2, Gal4 AD-p53 (1–52)/Gal4 BD-Mdm2 (1–125), or Gal4 AD-p53 (1–52)/Gal4 BD-Mdm2 (1–125)-M62A in non-selective medium were washed in water and inoculated at OD600 = 0.2 into selective medium containing DMSO or nutlin at the indicated concentrations. Growth of the cultures was monitored by recording the absorbance at 600 nm at the indicated time points. Data from a repeat experiment are depicted in Additional file 3: Figure S3

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