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Fig. 3 | BMC Biology

Fig. 3

From: A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53

Fig. 3

Nutlin is a substrate for the ABC transporter Pdr5. a Overnight cultures of either wild-type strain or the indicated deletion strains (pdr5Δ, pdr15Δ, pdr1Δ, etc.) containing plasmids encoding Gal4 AD-p53 (1–52)/Gal4 BD-Mdm2 were washed in water and inoculated at OD600 = 0.2 into selective medium containing DMSO or nutlin at the indicated concentrations. Growth of the cultures was monitored by recording the absorbance at 600 nm after 0, 24, 44, and 68 h following inoculation. Growth at 68 h is indicated in the plot. Additional file 5: Figure S5A contains the complete dataset and Additional file 6: Figure S6 contains data from a repeat experiment. b Overnight cultures of pdr5Δ cells containing plasmids encoding Gal4 AD-p53 (1–52)/Gal4 BD-Mdm2 and Pdr5 or Gal4 AD-p53 (1–52)/Gal4 BD-Mdm2 alone were washed in water and inoculated at OD600 = 0.2 into selective medium containing DMSO or nutlin at the indicated concentrations in duplicate. For both strains, growth as measured by average OD600 of duplicate cultures is plotted against time. Ends of the vertical bar indicate the OD600 values of the duplicate cultures

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