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Fig. 4 | BMC Biology

Fig. 4

From: A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53

Fig. 4

The ABC9Δ strain has increased permeability to several compounds. a Overnight cultures of either wild-type, ABC3Δ (pdr1Δ pdr3Δ pdr5Δ), or ABC9Δ (pdr1Δ pdr3Δ pdr5Δ snq2Δ pdr10Δ pdr11Δ pdr15Δ yor1Δ ycf1Δ) cells in rich medium (YEPD) were washed in water and inoculated at OD600 = 0.0625 into YEPD containing either DMSO, doxorubicin, cycloheximide, myriocin, or nutlin at the indicated concentrations in duplicate. For each strain, growth as measured by average OD600 of duplicate cultures after 40 h following inoculation was recorded. Percentage inhibition at different concentrations was calculated by normalizing with respect to the growth in DMSO-treated cultures. Ends of the vertical bar indicate values of percentage inhibition for the duplicate cultures. b Overnight cultures of ABC3Δ or ABC9Δ cells containing plasmids encoding either Gal4 AD-Csm1/Gal4 BD-Dsn1 or Gal4 AD-p53 (1–52)/Gal4 BD-Mdm2 in non-selective medium were washed in water and inoculated into selective medium at OD600 = 0.0625 Growth of the cultures was monitored by recording the absorbance at 600 nm after 0, 26, 44, and 68 h following inoculation. Data from a repeat experiment are shown in Additional file 7: Figure S7

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