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Fig. 5 | BMC Biology

Fig. 5

From: A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53

Fig. 5

Inhibition of p53 (1–52)/Mdm2 interaction by AMG232 and MI-773 is more efficient in ABC9Δ cells in comparison to wild-type and ABC3Δ cells. a Overnight cultures of either wild-type, ABC3Δ cells, or ABC9Δ cells containing plasmids encoding either Gal4 AD-Csm1/Gal4 BD-Dsn1 or Gal4 AD-p53 (1–52)/Gal4 BD-Mdm2 in non-selective medium were washed in water and inoculated at OD600 = 0.2 into selective medium containing DMSO or MI-773 at the indicated concentrations in duplicate. For each strain, growth as measured by average OD600 of duplicate cultures is plotted at different time points following inoculation (0, 19, 27, 44, and 51 h). Ends of the vertical bar indicate the OD600 values of the duplicate cultures. b Same as a, but performed with AMG232

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