Fig. 6
The full-length p53/Mdm2 interaction is more resistant to small-molecule inhibitors in comparison to the p53 (1–52)/Mdm2 interaction. a Overnight cultures of ABC9Δ cells containing plasmids encoding either Gal4 AD-Csm1/Gal4 BD-Dsn1, Gal4 AD-p53FL/Gal4 BD-Mdm2, or Gal4 AD-p53 (1–52)/Gal4 BD-Mdm2 in non-selective medium were washed in water and inoculated at OD600 = 0.2 into selective medium containing DMSO or nutlin at the indicated concentrations in duplicate. For each strain, growth as measured by average OD600 value of duplicate cultures is plotted at different time points following inoculation (0, 29, 46, 55, 71, and 77 h). Ends of the vertical bar indicate the OD600 values of the duplicate cultures. b, c Same as in a, but performed with MI-773 and AMG232, respectively