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Fig. 3. | BMC Biology

Fig. 3.

From: MultiBac: from protein complex structures to synthetic viral nanosystems

Fig. 3.

Flu polymerase structures. Crystal structures of MultiBac-produced polymerases of the three major influenza subtypes, fluA, fluB and fluC, have been determined [32,33,34,35]. For fluA and fluB, a polyprotein approach was used to balance stoichiometry. Subunits are expressed as a single open reading frame (ORF) encoding a large polyprotein, which is then processed by highly specific protease included in the ORF [18, 37]. By contrast, fluC was crystalized using conventional co-expression of the subunits. FluA and fluB polymerase in the crystals are bound to cognate RNA; the fluC structure represents the apo form of the complex. More recently, the molecular interactions between fluA polymerase and a short C-terminal domain (CTD) peptide from RNA polymerase II (Pol II) were elucidated [34] (left). The asterisk denotes the position of Pol II CTD peptides bound. PDB accession numbers are: fluA polymerase, PDB 5M3H; fluB polymerase, PDB 4WSA; fluC polymerase, PDB 5D9A

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