Skip to main content
Fig. 5. | BMC Biology

Fig. 5.

From: MultiBac: from protein complex structures to synthetic viral nanosystems

Fig. 5.

Mammalian multigene delivery and gene editing. The tropism of the MultiBac baculovirus was expanded by pseudotyping. A gene encoding for vesicular stomatitis virus glycoprotein (VSV-G) was integrated into the viral LoxP site, accompanied by a mCherry gene for tracking virus performance, resulting in the MultiBacMam virus (bottom right). MultiBacMam efficiently transduces mammalian cell types known to be recalcitrant to plasmid-based transfection, such as human umbilical vein endothelial cells (HUVECs), neurons and induced pluripotent stem (iPS) cells, with multigene DNA cargos encoding multiple fluorescently labeled proteins (top three rows). Transduction with MultiBacMam virus comprising the elements required for CRISPR/Cas9-mediated gene editing and a GFP-encoding gene as model DNA cargo resulted in successful integration into the HMG locus (bottom row) [63]

Back to article page
\