Fig. 4

Examples of expression profiles of genes with previously absent or incorrect gene models. The previous gene models originate from gene annotation v2.1 of the Volvox carteri genome available on the Volvox pages of the Phytozome V12 platform. a Example of a gene with an incorrectly predicted gene model: the gene model of gene Vocar.0007s0316 is not in accordance with the expression profiles at exon 5 (prediction too short), exon 6 (non-existent in reality), and the 5’- and 3’-UTRs (no prediction). b Example of another gene with an incorrectly predicted gene model: the gene model of gene Vocar.0001s0415 is not in accordance with the expression profiles at exons 11, 12, and 17 (prediction too short). There are also inconsistencies on the 5’-UTR (no prediction) and on the 3’-UTR (prediction somewhat too short). Moreover, there are in fact two additional exons between exons 17 and 18 (no prediction), and there is definitely no intron within the 3’-UTR. c Example of a gene with previously absent gene model: the gene is located on scaffold 4 at position 1,393,429 to 1,402,937. It codes for a protein with 837 amino acid residues. In all panels (a–c), the previous exon-intron predictions for these genes according to annotation v2.1 of the V. carteri genome are given directly below the expression profiles; exons are shown as blue bars and introns as thick gray lines. Thick red lines indicate differences between the previous prediction according to annotation v2.1 and our prediction. Our own exon-intron predictions for these genes are shown at the bottom; exons are illustrated as green bars and introns as thick gray lines. Our predictions are supported by the obtained expression profiles (single perfect matches) shown in dark blue