Fig. 6

Functional enrichment analysis of the most highly expressed genes. The 50 most highly expressed genes in somatic cells, reproductive cells, and in total were classified based on Pfam, GO, and PANTHER assignments of gene annotation v2.1 on the Phytozome V12 platform. Because several genes came without functional gene annotation, those genes were subject to individual BLASTP searches and the annotation of the hit with the highest sequence similarity was used for classification. In this way, all involved genes obtained a functional assignment. Functional groups that contained not more than one member in both cell types were combined in the group ‘other functions’ for reasons of clarity. a Classification of the most highly expressed genes in somatic cells. b Classification of the most highly expressed genes in reproductive cells. c Classification of the most highly expressed genes in total. d Venn diagram illustrating overlaps between the three groups