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Fig. 9 | BMC Biology

Fig. 9

From: Whole transcriptome RNA-Seq analysis reveals extensive cell type-specific compartmentalization in Volvox carteri

Fig. 9

Protein domain classification by molecular function followed by analysis for cell type-specific distribution of expression. All genes were screened for assigned Pfam, GO, and PANTHER identifiers regarding their molecular function and the identifiers were assigned to higher level GO-terms. The identified protein domains were sorted into groups and subgroups using QuickGO. The percentage share of each group and subgroup within the total number of protein domains with assigned functions was determined. Small groups or subgroups with a percentage share of less than 2% were combined for reasons of clarity (see ‘other…’). The very large groups ‘catalytic activity’ (44%) and ‘binding’ (37%) are shown with their subgroups. Each group or subgroup was analyzed for the proportion of protein domains with overexpression (fold difference in expression ≥ 2) in somatic cells or reproductive cells, or without distinct differences in expression between the two cell types. Small pie charts show the results. Yellow color: share of protein domains that are overexpressed in somatic cells; green color: share of protein domains that are overexpressed in reproductive cells; gray color: share of protein domains without cell type-specific overexpression. Groups and subgroups that differ clearly from the distribution of the total quantity (deviation ≥ 20%) are highlighted by colored arrowheads. Yellow arrowhead: larger proportion of domains with overexpression in somatic cells. Green arrowhead: larger proportion of domains with overexpression in reproductive cells

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