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Fig. 6 | BMC Biology

Fig. 6

From: Smad4 SUMOylation is essential for memory formation through upregulation of the skeletal myopathy gene TPM2

Fig. 6

TPM2 siRNA transfection blocks the facilitating effect of Smad4 SUMOylation on spatial learning and memory. a Animals received lenti-Flag-Smad4WT + control siRNA, lenti-Flag-Smad4WT-SUMO1 + control siRNA, or lenti-Flag-Smad4WT-SUMO1 + TPM2 siRNA (4 pmol) transduction and were subjected to water maze learning. n = 8 for each group, F(2,21) = 11.94, # P < 0.001; q = 6.58, # P < 0.001 comparing the lenti-Flag-Smad4WT + control siRNA and lenti-Flag-Smad4WT-SUMO1 + control siRNA groups; q = 5.12, **P < 0.01 comparing the lenti-Flag-Smad4WT-SUMO1 + control siRNA and lenti-Flag-Smad4WT-SUMO1 + TPM2 siRNA groups. The statistical difference between the lenti-Flag-Smad4WT-SUMO1 + control siRNA and lenti-Flag-Smad4WT-SUMO1 + TPM2 siRNA groups for a given trial is indicated by the significance sign: # P < 0.001. b Probe trial test. n = 8 for each group, F(2,21) = 5.33, *P < 0.05; q = 3.48, *P < 0.05 comparing the lenti-Flag-Smad4WT + control siRNA and lenti-Flag-Smad4WT-SUMO1 + control siRNA groups; q = 4.37, *P < 0.05 comparing the lenti-Flag-Smad4WT-SUMO1 + control siRNA and lenti-Flag-Smad4WT-SUMO1 + TPM2 siRNA groups. c Animals were sacrificed after the probe trial test and their CA1 tissue was subjected to TPM2 determination by western blotting. Lentivector transduction and expression were confirmed by western blotting using the anti-Flag antibody. d Quantified results of TPM2 expression. n = 8 for each group, F(2,21) = 268.4, # P < 0.001; q = 20.76, # P < 0.001 comparing the lenti-Flag-Smad4WT + control siRNA and lenti-Flag-Smad4WT-SUMO1 + control siRNA groups; q = 32.33, # P < 0.001 comparing the lenti-Flag-Smad4WT-SUMO1 + control siRNA and lenti-Flag-Smad4WT-SUMO1 + TPM2 siRNA groups. Data are expressed as means ± SEMs. IB immunoblotting, IP immunoprecipitation, SEM standard error of the mean

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