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Fig. 8 | BMC Biology

Fig. 8

From: Smad4 SUMOylation is essential for memory formation through upregulation of the skeletal myopathy gene TPM2

Fig. 8

The TPM2E122K mutant decreases TPM2 expression and impairs spatial learning and memory formation. a Flag-vector, Flag-TPM2WT, and various Flag-TPM2 mutant plasmids were transfected to HEK293T cells and TPM2 expression was determined by western blotting. Western blotting with anti-Flag antibody was used to confirm similar amounts of transfection and expression for each plasmid. b Quantified result of TPM2 expression. n = 5 for each group, F(7,32) = 9.09, P > 0.05; q = 7.25, # P < 0.001 comparing the TPM2E122K and control groups; and q = 9.91, # P < 0.001 comparing the TPM2E122K and TPM2WT groups. Raw data and statistics are provided as Additional file 9. c Animals received lenti-Flag-vector, lenti-Flag-TPM2WT, or lenti-Flag-TPM2E122K mutant vector transduction to the CA1 area of their brains and were subjected to water maze learning. n = 8 for each group, F(2,21) = 19.17, # P < 0.001; q = 8.22, # P < 0.001 comparing the TPM2E122K and control groups; and q = 6.73, # P < 0.001 comparing the TPM2E122K and TPM2WT groups. The statistical difference between the lenti-Flag-TPM2WT and lenti-Flag-TPM2E122K groups for a given trial is indicated by the significance signs: *P < 0.05, **P < 0.01 and # P < 0.001. d Probe trial test. n = 8 for each group, F(2,21) = 11.09, # P < 0.001; q = 4.34, *P < 0.05 comparing the TPM2E122K and control groups; and q = 6.55, **P < 0.01 comparing the TPM2E122K and TPM2WT groups. A representative swim pattern from each group is also shown. Data are expressed as means ± SEMs. IB immunoblotting, IP immunoprecipitation, SEM standard error of the mean

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