Fig. 3

The pangenome of Zymoseptoria tritici. a Venn diagram of the singleton, accessory, and core genes of the pangenome. b Categorization of the pangenome into singleton, accessory, and core genes according to the number of times a specific gene was identified among genomes. c Genes of the isolate IPO323 classified as either accessory or singleton in the pangenome were analyzed for clustering in the genome (black bar). In comparison, the mean of 1000 randomly drawn distributions of accessory genes are shown with grey bars. Accessory genes were significantly more likely to be in clusters than expected by chance (Fisher exact test, P < 0.0001). d Estimation of the sizes of the core genome (genes shared among all isolates) and the pangenome (all genes). Genomes were resampled in all possible combinations of 1–5 and the number of core and pangenome genes are reported as dots. The pangenome curve was modelled by fitting the power law regression formula y = Ax^B + C and the core genome curve was modelled by fitting the exponential regression formula y = Ae^Bx + C