Fig. 5

A time-resolved visualisation for the coral holobiont comparing the response of (a) Acropora millepora and (b) Stylophora pistillata to thermal stress over time (days). The graphical illustration of the changes to host and symbiont traits over time was constructed using Adobe Illustrator (CS6, Adobe Systems Software Ireland Ltd., CA, USA), where data points represent the magnitude of change (Fig. 4) for each trait that was significantly different from day 1 and the control at each time point (according to the PERMANOVA output). Lines are hand drawn interpolations of the change between time points with shading used to group host and symbiont responses to assist with interpretation. Photophysiological parameters (red solid lines and shading), host (pink solid lines and shading) and symbiont (green solid lines and shading) antioxidants (superoxide dismutase (SOD), glutathione (GSx) and catalase (CAT)/CAT-like activity), Symbiodinium pigmentation (including Symbiodinium density and chlorophyll a; orange dotted lines and shading), gross photosynthesis (blue solid line), dimethylsulfoniopropionate (DMSP) concentration (black solid line) and dimethyl sulfoxide (DMSO; black dotted line) are shown. The relative change is indicated by the left y-axis, note the change in scale for S. pistillata (b), where maximum relative change is 3. The time axis extends to day 20 for A. millepora because maximum/effective quantum yields were measured until day 20, whereas S. pistillata was more sensitive to thermal stress and measurements were not detected past day 15