Skip to main content
Fig. 6 | BMC Biology

Fig. 6

From: Memory retrieval in addiction: a role for miR-105-mediated regulation of D1 receptors in mPFC neurons projecting to the basolateral amygdala

Fig. 6

Effect of chronic morphine on miR-105 and influence of miR-105 inhibitor on D1 receptor expression in glutamatergic projection neurons from the mPFC to the BLA. a Left panel: neurons from the mPFC to the BLA labeled with fluorescent microspheres. Middle panel: harvesting the cytoplasmic contents of the labeled neuron via a patch pipette under visual control. Scale bar, 5 μm. Right panel: The averaged miR-105 expression in microsphere-labeled neurons in the mPFC in saline and morphine groups by single-cell RT-PCR (n = 5, *P < 0.05, compared to saline group). b Left panel: D1R staining (column 1, red color), eGFP labeling (column 2, green color), glutamatergic terminal marker VGLUT2 immunoreactivity (column 3 blue color), and coexpression of D1R, BDA, and VGLUT2 (column 4, white color) in slices of the BLA in miR-105 inhibitor NC-LV and miR-105 inhibitor-LV group. Right panel: The average optical density of D1R in glutamatergic terminals of projection neurons from the mPFC to the BLA in miR-105 inhibitor NC-LV and miR-105 inhibitor-LV groups (n = 5, *P < 0.05, compared to miR-105 inhibitor NC-LV group). The figure insets are the magnified views marked with small white rectangles; scale bar, 5 μm. In the insets of higher magnified areas the scale bar is 1 μm. Data are shown as the mean ± SEM

Back to article page