Fig. 7
Effect of D1 receptor agonist SKF38393 on mPFC-to-BLA glutamatergic synaptic transmission under chronic morphine or saline conditions. a Left panel: Images of coronal brain slice showing expression of ChR2–mCherry (red color) 6 weeks after virus injection into the mPFC. Scale bar, 500 μm. Middle panel: Images of coronal brain slice showing strong ChR2–mCherry-positive fibers from the mPFC in the BLA 6 weeks after virus injection into the mPFC. Scale bar, 500 μm. Right panel: Light-evoked EPSCs recorded from BLA neurons after optical stimulation of mPFC-to-BLA fibers before and after bath application of DNQX. b Left panel: Representative traces recorded from BLA neurons after optical stimulation of mPFC-to-BLA fibers before and after bath application of 10 μM SKF38393 in response to 5 ms light pulses. Middle panel: Time course of light-evoked EPSC from BLA neurons before and after 10 μM SKF38393 in saline (n = 8 from 5 mice) and chronic morphine (n = 8 from 5 mice) group. Right panel: The averaged percentage of the effect of 10 μM SKF38393 on light-evoked EPSC in saline (n = 8 from 5 mice) and chronic morphine (n = 8 from 5 mice) groups. *P < 0.05, compared to before SKF38393. c Left panel: Representative traces recording of PPF from BLA neurons after a paired-pulse optical stimulation protocol before and after bath application of SKF38393 in saline and chronic morphine groups. Middle panel: Averaged PPF before and after 10 μM SKF38393 in saline group (n = 5 from 3 mice). Right panel: Averaged PPF before and after 10 μM SKF38393 in chronic morphine group (n = 5 from 3 mice). *P < 0.05, compared to before SKF38393. Data are shown as the mean ± SEM