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Fig. 3 | BMC Biology

Fig. 3

From: Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells

Fig. 3

Recruitment of xenophagy markers to Mtb in human lymphatic endothelial cells is impaired when PDIMs are absent. Quantification of immunofluorescent analysis of three proteins involved in xenophagy: a ubiquitin (Ub), b p62 and c NDP52. The process of infection, immunofluorescent labelling, imaging and quantification was identical to that explained in Fig. 1. At least six fields of view were imaged per sample per replicate and three independent replicates were performed. The total number of analysed Mtb particles is also displayed for each condition (N). The overall mean is shown with error bars representing the standard error of the mean. Statistical significance was determined using one-way ANOVA with Tukey’s post-test. Mtb Mycobacterium tuberculosis, ns not significant, PDIM phthiocerol dimycocerosate, Ub ubiquitin, WT wild type, *** p < 0.001, * p < 0.05

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