Fig. 1
eNOS is upregulated in intestinal tumors from Apcfl/fl, Apcfl/+, and Apcfl/+ Ptenfl/+ mouse models. a eNOS and iNOS immunohistochemistry of wild-type, Apcfl/fl, Apcfl/+, and Apcfl/+ PTENfl/fl mice intestinal tissues. eNOS expression was absent in the wild-type intestine whereas Apcfl/fl sections showed intense immunostaining in the hyperproliferative crypts. In the Apcfl/+ and Apcfl/+ PTENfl/+ mice models, hyperproliferative areas resulted in upregulation of eNOS (black arrows) whereas normal intestinal tissue did not show expression of this NOS isoform (red arrow). The bottom of normal crypts did not show staining of eNOS (gray arrows). iNOS expression was absent in all three models. Scale bars: 100 μm and 200 μm. b RT-qPCR analysis of eNOS expression from wild-type, Apcfl/+ and Apcfl/+ PTENfl/+ tissue. * P < 0.05 compared with the wild-type non-proliferative tissue. eNOS endothelial NO synthase, iNOS inducible NO synthase, NOS NO synthase, RT-qPCR quantitative real-time reverse-transcriptase polymerase chain reaction, Wt wild type