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Fig. 4 | BMC Biology

Fig. 4

From: A role for endothelial nitric oxide synthase in intestinal stem cell proliferation and mesenchymal colorectal cancer

Fig. 4

eNOS is upregulated in organoids derived from Apcfl/fl mice intestine and NO trapping decreases their proliferation and downregulates the expression of stem-cell markers. a Representative images from typical wild-type and Apcfl/fl organoids. Scale bars: 400 μm (left) and 200 μm (right). b RT-qPCR analysis of iNOS and eNOS expression from wild-type and Apcfl/fl organoids. *P < 0.05 compared with the wild-type organoids. c and d Proliferation assay of control or wild-type and Apcfl/fl organoids treated for 48 hours with c-PTIO (500 μM). Proliferation was determined by image analysis with ImageJ software. *P < 0.05 compared with the control. Scale bars: 1000 μm. e RT-qPCR analysis of Lgr5, Troy, Vav3, and Slc14a1 expression from control or c-PTIO (500 μM) treated wild-type and Apcfl/fl organoids. eNOS endothelial NO synthase, iNOS inducible NO synthase, RT-qPCR quantitative real-time reverse-transcriptase polymerase chain reaction, WT (wt, Wt) wild-type

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