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Fig. 6 | BMC Biology

Fig. 6

From: A role for endothelial nitric oxide synthase in intestinal stem cell proliferation and mesenchymal colorectal cancer

Fig. 6

NO trapping with c-PTIO reduces the capacity of mesenchymal HCT-116 cells to form tumors in xenografted mice and decreases expression in vivo of β-catenin and Bmi1. a Control or HCT-116 cells pre-treated with c-PTIO (100 μM) for 24 hours were subcutaneously injected into the flanks of immunocompromized mice. Final tumor volumes were analyzed after 37 days of inoculation. Mice xenografted with HCT-116 cells were treated with vehicle or c-PTIO (320 mg/kg) once a week. b Mice weights were monitored over time. c Tumor size was measured during the course of treatment. d and e Final tumor volume was analyzed after harvesting. f Representative images from immunohistochemistry of eNOS and iNOS from HCT-116-derived tumors and β-catenin and Bmi1 from vehicle or c-PTIO treated mice tumors. *P < 0.05, **P < 0.01, ***P < 0.001 compared with control. eNOS endothelial NO synthase, iNOS inducible NO synthase

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