Table 2 Yields of sequences using different extraction and analysis methods. The percentage of the raw reads that were included in the output of the two methods (MinKNOW and Canu v1.4, or Albacore and Canu v1.5) is shown together with the total sequence lengths and the number of reads exceeding the indicated lengths. See ‘Methods’ for experimental details and Additional file 1: Table S1 for more information
From: De novo assembly of the complex genome of Nippostrongylus brasiliensis using MinION long reads
| Â | Yield | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Preparation Method | Raw reads | MinKNOW | Albacore | ||||||
% | Gb | >50Â kb | >100Â kb | % | Gb | >50Â kb | >100Â kb | ||
1. Sonication, DNeasy | 930,957 | 99.9 | 2.375 | 80 | 15 | 99.1 | 2.603 | 472 | 82 |
2. Sonication, G2, Tip20 | 1,845,520 | 85.2 | 2.289 | 2252 | 17 | 98.0 | 3.002 | 4888 | 1006 |
3. Direct lysis, DNeasy | 451,578 | 99.9 | 1.723 | 179 | 26 | 98.8 | 2.028 | 1710 | 775 |
4. Pipette squashed, G2, Tip20 | 2,333,064 | 83.9 | 3.834 | 10 | 0 | 98.4 | 4.678 | 427 | 89 |