Fig. 4

Quantification of RNAi-induced phenotypes. The worms were imaged during the first day of adulthood. The values for each worm were divided by the mean value of the corresponding control group. Each dot represents an individual worm. Bars represent mean ± standard deviation (****p < 10^–4, **p < 10^–2, one-way analysis of variance (ANOVA) test with Bonferroni post hoc correction). a Quantification of the Dumpy phenotype following dpy-11 RNAi treatment. N2 worms (upper panel) or rrf-3 mutants (lower panel) were fed with bacteria expressing an empty vector control or dsRNA complementary to dpy-11. b Quantification of fluorescence intensity in whole animals. EG7841 worms expressing mCherry in all somatic cells were fed with bacteria expressing either an empty vector control or dsRNA complementary to mCherry. The RNAi-producing bacteria were grown to the indicated optical density (O.D.). P0 condition: The eggs were laid on RNAi-producing bacteria lawns. F1 condition: The progeny of the RNAi-treated worms that were laid and grown on standard OP50 bacteria