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Fig. 4 | BMC Biology

Fig. 4

From: Alarm pheromone and kairomone detection via bitter taste receptors in the mouse Grueneberg ganglion

Fig. 4

Molecular bitter taste signalling elements are expressed in the mouse Grueneberg ganglion. a RT-PCR experiments revealing expression of three bitter taste receptors (Tas2r115, Tas2r131 and Tas2r143) and Gnat3 transcripts in the tongue (T) and in the mouse Grueneberg ganglion (GG). b Tas2r115, Tas2r131, Tas2r143, Gnat3 and B2m expression in the GG and tongue of mice. + cDNA sample, – negative control omitting the reverse transcription. c Transcripts of sweet and umami taste receptors (Tas1r1, Tas1r2 and Tas1r3), phospholipase-C β2 (Plcβ2) and of the transient receptor potential channel M5 (Trpm5) were found in the tongue, but not in the GG. d Immunohistochemistries on mice tongue tissue section (circumvallate papillae) with antibodies against TAS2R143 and GNAT3. e Immunohistochemistries on mice GG sections with antibodies against TAS2R143 and GNAT3. GG cells expressing GFP are visible in green due to the intrinsic expression of the GFP. In ac, M is 100-bp ladder and H is H2O. In d, e, white arrowheads correspond to enlarged views displayed in insets; nuclei are counterstained in blue with 4’,6-diamidino-2-phenylindole (DAPI); scale bars are 20 μm

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