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Fig. 6 | BMC Biology

Fig. 6

From: Alarm pheromone and kairomone detection via bitter taste receptors in the mouse Grueneberg ganglion

Fig. 6

TAS2R143 is a narrowly tuned GG ligand receptor. a Bar graph representing the response rate induced in HEK cells transfected respectively with TAS2R115, TAS2R131 and TAS2R143. Superimposed insets display a representative successful transfection due to GFP expression and Fura-2 AM uptake measured at 380 nm in a colour-coded map. For TAS2R115, 2-ethylpyrazine, 2-EP (8/144); 2-sec-butylthiazoline, SBT (0/310); 2-propylthietane, 2-PT (0/104); 2,4,5-trimethylthiazoline, TMT (2/205); 2,4-lutidine, 2,4-Lu (0/112); 2,6-dimethylpyrazine, 2,6-DMP (0/65). For TAS2R131, 2-EP (7/147), SBT (2/203), 2-PT (0/333), TMT (0/248), 2,4-Lu (0/183), 2,6-DMP (0/171). For TAS2R143, 2-EP (20/146), SBT (45/345), 2-PT (112/237), TMT (9/236), 2,4-Lu (0/58), 2,6-DMP (0/84). b Representative calcium transients induced by the bitter tastant 2-EP as well as by the alarm pheromone SBT and by the kairomones 2-PT, TMT, 2,4-Lu and 2,6-DMP at (100 μM) and by a control pulse of ATP (100 μM) on HEK cells transfected with TAS2R143, TAS2R131 and TAS2R115 respectively. c Detail of the pseudo dose response obtained for TAS2R143 and its responding ligands from a. In a, scale bars = 15 μm; n/x = number of cells responding/number of cells tested and viable. In b, fluorescence intensity Fura-2 ratio = F340/F380 is indicated by arbitrary units (a.u.); time is indicated by a horizontal bar. In c, the tested concentrations are indicated by coloured dots (2-PT in red, SBT in purple, 2-EP in blue and TMT in green); as a minimum, 58 viable cells were used for each cue and concentration tested

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