TY - JOUR AU - Shen, Yi AU - Dana, Hod AU - Abdelfattah, Ahmed S. AU - Patel, Ronak AU - Shea, Jamien AU - Molina, Rosana S. AU - Rawal, Bijal AU - Rancic, Vladimir AU - Chang, Yu-Fen AU - Wu, Lanshi AU - Chen, Yingche AU - Qian, Yong AU - Wiens, Matthew D. AU - Hambleton, Nathan AU - Ballanyi, Klaus AU - Hughes, Thomas E. AU - Drobizhev, Mikhail AU - Kim, Douglas S. AU - Koyama, Minoru AU - Schreiter, Eric R. AU - Campbell, Robert E. PY - 2018 DA - 2018/01/16 TI - A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578 JO - BMC Biology SP - 9 VL - 16 IS - 1 AB - Genetically encoded calcium ion (Ca2+) indicators (GECIs) are indispensable tools for measuring Ca2+ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to green fluorescent protein-based GECIs due to the longer wavelength light used for excitation. Longer wavelength light is associated with decreased phototoxicity and deeper penetration through tissue. Red GECI can also enable multicolor visualization with blue- or cyan-excitable fluorophores. SN - 1741-7007 UR - https://doi.org/10.1186/s12915-018-0480-0 DO - 10.1186/s12915-018-0480-0 ID - Shen2018 ER -