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Fig. 5 | BMC Biology

Fig. 5

From: A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578

Fig. 5

Performance of K-GECO1 in organotypic brain slices. a K-GECO1 labeling of the soma and dendrites of neurons in the ventromedial nucleus (VMN) of organotypically cultured newborn rat hypothalamus slices. b ATP-induced cytosolic Ca2+ rise in VMN neurons. c Fluo-4 AM loaded and K-GECO1 transfected into VMN slice. d Representative fluorescence intensity traces of ATP treatment causing a Ca2+ rise, as reported by both Fluo-4 AM and K-GECO1. Supporting numeric data are provided in Additional file 11. ATP adenosine triphosphate, VMN ventromedial nucleus

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