Fig. 6
In vivo imaging of K-GECO in zebrafish Rohon–Beard cells. a Schematic setup of the experiment. b Image of Rohon–Beard cells expressing K-GECO1 with region of interest (ROI) indicating cytoplasm. c K-GECO1 Ca2+ response to pulse stimuli in the cytosol. d K-GECO1 Ca2+ response to pulse stimuli in the nucleus. e Fluorescence fold change of K-GECO1 and f jRGECO1a under various numbers of pulses. g Half decay time of K-GECO1 and h jRGECO1a under various numbers of pulses. Supporting numeric data are provided in Additional file 12