Fig. 6

Effect of time after directed differentiation from ISC on mRNA expression of TJ proteins from ENT (a) and GOB (b) organoids. Within each gene, all treatments are normalized to day 1 (D1) post-differentiation for both ENT and GOB organoids. Data were analyzed using one-way ANOVA (see text for P values). a Cldn2 (n = 3 for ENTs D1 to D3), Cldn7 (n = 3 for D1 and n = 2 for D2 as well as D3), Ocln (n = 3 for D1 as well as D3, and n = 2 for D2), and Tjp1 (ZO-1) (n = 3 for all). b Cldn2 (n = 3 for GOBs D1 to D3), Cldn7 (n = 3 for D1 and n = 2 for D2 as well as D3), Ocln (n = 3 for all), and Tjp1 (ZO-1) (n = 3 for GOB D1 as well as D3, and n = 2 for D2). Trends in mRNA expression of TJ proteins during directed differentiation from ISC to ENT or GOB organoids matched those depicted in Fig. 2a,b. Supporting data sets are deposited in the figshare repository [50]. Cldn claudin, D day, ENT enterocyte, GOB goblet cell, ISC intestinal stem cell, Ocln occludin, TJ tight junction, Tjp or ZO-1 tight junction protein