Fig. 1

ADMP restricts the size of the organizer domain. ADMP overexpression was induced by injection of increasing amounts of capped RNA (32–800 pg/embryo). Embryos were collected during early gastrula and analyzed for changes in gene expression patterns by qPCR and in situ hybridization. a, b qPCR analysis of the expression levels of dorsal (a) and ventral (b) genes. c–j Embryos were injected in dorsal or ventral blastomeres with ADMP mRNA (100 pg/embryo) together with a lineage tracer (turquoise) followed by in situ hybridization analysis with chordin (c–f) and gsc (g–j) probes to determine the effect on their expression domain. The relative arc of the expression domain was determined. f, j Graphs summarizing all the measurements of the relative sizes (%) of the expression domains. Chordin analysis: control, n = 75; ADMP dorsal, n = 46; ADMP ventral, n = 33, and gsc analysis: control, n = 16; ADMP dorsal, n = 19; ADMP ventral, n = 21. Statistical test; Dunnett’s analysis of variance (ANOVA) multiple comparisons test. *p < 0.05, ***p < 0.001, ****p < 0.0001, ns not significant