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Fig. 6 | BMC Biology

Fig. 6

From: ADMP controls the size of Spemann's organizer through a network of self-regulating expansion-restriction signals

Fig. 6

Blocking the ALK1 receptor preferentially blocks the anti-organizer activity of ADMP. ad, i Embryos were injected with RNA encoding the tALK1, tALK2, and tALK3 dominant negative receptors. During early gastrula stages the changes in the chordin expression domain were determined by in situ hybridization. eh, j To determine the receptor mediating the organizer-repressive activity of ADMP, four-cell-stage embryos were injected in a single dorsal blastomere with ADMP mRNA (50 pg/embryo) alone or together with one of the dominant negative type I receptors, tALK1, tALK2, or tALK3 (320 pg/embryo). Also, fluorescein isothiocyanate (FITC)-dextran was included as a lineage tracer (turquoise). At the onset of gastrulation, the changes in the size of the chordin expression domain were studied. The embryos were analyzed for the expression domain changes induced by ADMP gain of function and the extent of rescue by the different dominant negative receptors. The relative (%) arc of the expression domain was determined. Comparative truncated receptor analysis: control, n = 24; tALK1, n = 24; tALK2, n = 15; tALK3, n = 21, and block ADMP ventral activity analysis: control, n = 18; ADMP, n = 21; +tALK1, n = 16; +tALK2, n = 21; tALK3, n = 14. p values were calculated compared to controls (i) except in the rescue experiments that were compared to the ADMP sample (j). Statistical test; Dunnett’s (ANOVA) multiple comparisons test (j) and two-tailed t test (i). **p < 0.01, ****p < 0.0001, ns not significant

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