Fig. 1
Disruption of ETH action on octopaminergic neurons causes egg retention in virgin females. a Number of mature eggs retained in ovaries of isolated adult virgin females with Inka cells ablated (ETH-Gal4;Tubulin-Gal80ts/UAS-Reaper) (n = 30), blocked (ETH-Gal4/UAS-Shits) (n = 35), and EcR-silenced (ETH-Gal4;Tubulin-Gal80ts/UAS-EcR-RNAi) (n = 50) (biological replicates). b Co-staining of ETHR positive neurons in the vicinity of the juncture of ovaries (yellow arrow) and oviduct (purple arrowhead) ETHR-Gal4/UAS-mCD8-GFP (green), anti-TDC2 (red), and 4’,6-diamidino-2-phenylindole (DAPI, blue). Scale bar = 100 μm. c Number of mature eggs retained in the ovaries of virgin females with ETHR silenced in all octopaminergic neurons (Tdc2-Gal4) (n = 50) and in the neurons innervating the female reproductive tract (Bwktqs-Gal4) (n = 40) (biological replicates). Error bars represent standard error of the mean (SEM). ***p < 0.001, ****p < 0.0001