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Fig. 2 | BMC Biology

Fig. 2

From: Stress-induced reproductive arrest in Drosophila occurs through ETH deficiency-mediated suppression of oogenesis and ovulation

Fig. 2

ETH mobilizes calcium in octopaminergic neurons and evokes contractions of the ovarian peritoneal sheath. a, a’, a” Calcium imaging of octopaminergic neurons innervating excised ovary (red arrowheads) and oviduct (blue arrowheads) of mated females (genotype Tdc2-Gal4/UAS-GCaMP6S). Fluorescence responses observed in neurons innervating the oviduct (blue arrowheads) before (a) and after (a’ and a’ inset) ETH1 exposure (10 μM). Change in fluorescence before and after ETH application is indicated by the horizontal blue arrow. Fluorescence responses recorded at the base of the ovary (a”, orange arrowhead) after ETH application (vertical orange arrow in a” inset); scale bar = 100 μm; insets are 2X (a’) and 1.5X (a”). b Nerve terminal fluorescence intensity from a over time, before and after treatment, indicated by black arrow. c, d Contractile responses of ovaries excised from Canton-S females before (blue, c) and after ETH (green, c) or octopamine (red, d) treatment at various concentrations. Statistics indicate difference in contraction frequency between agonist and saline (n = 20). e Ovarian contractile responses following ETHR silencing in octopaminergic neurons (TDC2-Gal4/ETHR-IR2) and genetic controls before and after ETH1 (10 μM) treatment indicated by the black arrowhead and dotted line (n = 25) (biological replicates). f Contraction rate of each genotype before and after ETH1 treatment is compared statistically among genotypes or across treatment. Contraction frequency for each genotype was compared both to its contraction frequency before and after ETH treatment (Student’s t test) and to other genotypes either before or after ETH treatment (one-way analysis of variance (ANOVA)). Error bars represent SEM. NS, p > 0.05; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. TDC2-Gal4/+: Red, UAS-ETHR-IR2/+: Green, UAS-ETHR-IR2/TDC2-Gal4: Blue

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