Fig. 5

ETH activates ovulation in vivo. a Scoring system for in vivo ovulation. Ovaries were dissected, and oocyte position was scored according to which physiological plane was broken by the tip of the furthest oocyte (scale bar = 100 μm). b Quantification of oocyte distance in oviducts of females 90 min after injection with saline (blue bars), ETH (green bars), or OA (pink bars) (n = 20) (biological replicates). c Percent of the same females with an egg present in their bursa (percent ovulated) (n = 20) (biological replicates). Error bars represent SEM. *p < 0.05, **p < 0.01, ****p < 0.0001