Skip to main content
Fig. 1 | BMC Biology

Fig. 1

From: Sox5 is involved in germ-cell regulation and sex determination in medaka following co-option of nested transposable elements

Fig. 1

Comparative analysis of the dmrt1a and dmrt1bY co-ortholog promoters and presence of Rex1 element sequences in the genomes of selected fish species. a Comparative analysis of the medaka dmrt1 co-ortholog promoter regions. Differences in length for the two promoter regions are caused by Rex1 and Izanagi transposable elements as well as repeats 3 and 4 that were inserted into the dmrt1bY promoter after the duplication event that gave rise to the dmrt1bY gene approximately 10 million years ago [17]. Regions α, β, or γ (brackets [] underlined in red) contain multiple Sox5 binding sites within Rex1, Izanagi, and repeat 3, respectively, that have been subjected to chromatin immunoprecipitation (ChIP) (see also Additional file 1: Figure S1). The red star (*) identifies the Dmrt1 binding site described in [17]. b Alignment of the Y-chromosomal Rex1 element together with the 19 remaining Rex1 copies encompassing the sox5 binding site in the medaka genome. Dots indicate conserved nucleotides. Black arrows define primers used for chromatin immunoprecipitation. c Presence of Rex1 element (i) partial sequences, (ii) sequences encompassing the dmrt1bY-nested sox5 binding site, and (iii) sequences encompassing the dmrt1bY-nested sox5 binding site with the intact sox5 binding site in the genomes of medaka (Oryzias latipes), tilapia (Oreochromis niloticus), zebrafish (Danio rerio), cavefish (Astyanax mexicanus), cod (Gadus morhua), gar (Lepisosteus oculatus), stickleback (Gasterosteus aculeatus), platy (Xiphophorus maculatus), Amazon molly (Poecilia formosa), fugu, tetraodon, and coelacanth (Latimeria chalumnae)

Back to article page