Fig. 11

RhoH depletion alters PAK2 localisation and PAK2-induced lamellipodia. PC3 cells were transfected with the indicated siRNAs. After 48 h, cells were transfected with empty vector or vector encoding myc-PAK2. Cells were fixed 24 h later and stained for F-actin with Alexa Fluor 546 phalloidin, anti-myc antibodies for Myc-PAK2 and DNA (DAPI). Representative images of cells are shown. Scale bar 10 μm. Arrows indicate protrusions with no PAK2 or in which PAK2 does not overlap with F-actin