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Fig. 4 | BMC Biology

Fig. 4

From: An RNAi screen of Rho signalling networks identifies RhoH as a regulator of Rac1 in prostate cancer cell migration

Fig. 4

RhoH is expressed in a subset of cancer cell lines and correlates with poor prognosis in prostate cancers. a, b Several epithelial cancer cell lines express RhoH. a RNA was analysed by reverse transcription (RT)-PCR to determine which cell types express mRNA for RhoH, RhoA and Rac2. GAPDH was used as a control. Gel is representative of three experiments. b RNA was analysed by qPCR for RhoH and Rac1 expression and normalised to GADPH mRNA. mRNA levels for each cell type are shown relative to levels in PC3 cells. Mean +/− standard deviation for the values of three technical replicates. c Expression of RhoH in prostate cancer correlates with poor prognosis. Publicly available RNA expression data from 140 prostate cancers from the Memorial Sloan Kettering Cancer Center (GSE21032) were analysed for RhoH expression. Samples were divided into those with high or low RhoH expression (see Methods). Kaplan-Meier curves show recurrence-free survival (RFS) measured by prostate-specific antigen (PSA) levels, indicating that high RhoH expression correlates with shorter RFS. Numbers under the graph show the number of patients without a recurrence event at a given time point, indicating that high RhoH expression correlates with earlier relapse and shorter time to relapse

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