Fig. 5

Analysis of the screening data. a Density plot (frequency distribution) of the negative controls (control(RNAi)) and positive controls (atfs-1(RNAi)) based on the GFP intensities. b Fold change (FC) of GFP intensity of the 1207 tested RNAi clones against the ordered index. Genes with a P value < 0.001 and FC < 0.66 or FC > 1.5 are considered as candidates. Depletion of 208 RNAi clones downregulates Phsp-6::GFP signal, whereas only one candidate triggers a further induction of the reporter. c Interaction network of the 208 genes whose depletion reduces the UPR^mt. Networks are built using STRING [76], based on predicted and described interactions in different organisms. Nodes are proteins, and the edges represent the associations between nodes. Clusters show genes involved in processes previously described to be involved in the regulation of the mitochondrial stress response such as ribosome, proteasome, RNA processing, protein transport and complex I of the mitochondrial electron transport chain. d Brightfield and green images of control(RNAi) and nuo-6(RNAi). Depletion of nuo-6 triggers a developmental delay in addition to the reduction in the UPR^mt reporter expression. e FC of worm length of the 1207 tested RNAi clones against the ordered index. Genes with an FC < 0.85 are considered as clones affecting development. Depletion of 303 genes reduces size of the worms. f Interaction network of the 303 genes whose depletion reduces size of the worms. Networks are built using STRING [76], based on predicted and described interactions in different organisms. Nodes are proteins; edges represent the associations between nodes. Clusters show genes involved in DNA replication and repair, fatty acid metabolism, ion channels and nuclear pore complex