Fig. 1
From: The integrated stress response regulates BMP signalling through effects on translation
Depletion of dPPP1R15 or dGCN2 alters wing venation. a Representative photomicrographs (5× objective) of adult wings of the indicated genotypes. Lower panels are enlargements of the crossvein territories: anterior crossvein (ACV) (open arrowhead) and posterior crossvein (PCV). Note extra venation (closed arrowheads) in wings expressing gcn2 RNAi. Scale bars = 250 μm. b Quantification of ACV phenotypes. For brevity, enGAL4 > UAS-ppp1r15 RNAi is indicated as en > ppp1r15 RNAi. enGAL4 > UAS-gcn2 RNAi is indicated as en > gcn2 RNAi. n denotes number of animals counted. P values calculated using X2 statistics with Bonferroni correction for multiple comparisons. c Representative photomicrographs of adult wings (5× objective) of the indicated genotypes. en > dicer2 indicates enGAL4 > UAS-dicer2. en > dicer2;ppp1r15 RNAi indicates enGAL4 > UAS-dicer2;ppp1r15 RNAi. Lower panels are enlargements of the crossvein territories. Scale bars = 250 μm. d, e Representative photomicrographs of adult wings (5× objective) of the indicated genotypes. nab > gcn2 RNAi indicates enGAL4 > UAS-gcn2 RNAi. dppd5/+; nab > gcn2 RNAi indicates Dppd5/+; nab > UAS-gcn2 RNAi. Lower panels are enlargements of the crossvein territories. Note extra venation (closed arrowheads). f Quantification of wings from d and e. Scale bars = 250 μm. g Representative photomicrographs of adult eyes (dorsal view) of the indicated genotypes; inset shows zoom of eye. Scale bar = 200 μm