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Fig. 1 | BMC Biology

Fig. 1

From: A Go-type opsin mediates the shadow reflex in the annelid Platynereis dumerilii

Fig. 1

Wavelength dependence of the adult Platynereis shadow response and impaired shadow reflex in Go-opsin1−/− animals. a Schematic of light regime used for the Shadow Reflex Assay. Following a 60 min acclimatisation period of constant light, 12 ‘shadow stimuli’, consisting of 2 s periods of abrupt darkness, occur between 60 s intervals of light. Stimulation of foraging behaviour refers to the addition of Spinach-conditioned artificial seawater 15 min prior to the first shadow (see also Additional file 1: Movie S1 and Additional file 2). b Efficiency of the Shadow Reflex in wildtype adult Platynereis dumerilii subjected to shadows under white light (black, n = 74) and five different monochromatic light conditions, 400 nm (purple, n = 74), 470 nm (blue, n = 74), 500 nm (cyan, n = 74), 520 nm (green, n = 56) and 590 nm (orange, n = 44). Whiskers in the box plot indicate the range of scores; means are indicated with a cross whilst individual scores are plotted as diamonds. Data from 7 experimental days are combined. c Efficiency of the Shadow Reflex in sibling Go-opsin1+/+ (black, n = 30), Go-opsin1Δ8/+ (blue, n = 30) and Go-opsin1Δ8/Δ8 (red, n = 30) adult Platynereis dumerilii under five different light conditions. Whiskers in the box plots indicate the range of scores; means: cross, individual scores: diamonds. Significance scores (** < 0.01, *** < 0.001, **** < 0.0001) represent P values of Mann–Whitney U tests comparing wildtype, heterozygous and homozygous siblings

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