Fig. 1

InsP₃R-modulated Ca²⁺ is required for C. albicans phagocytosis. a Confocal images depicting the changes of cytosolic Ca²⁺ concentrations ([Ca²⁺]_c) in BMDMs incubated with C. albicans. The upper line depicts individual cellular [Ca²⁺]_c values. The upper histogram depicts the summary of peak [Ca²⁺]_c changes during phagocytosis (n = 32). Representative snapshots of cytoplasmic Ca²⁺ images were taken at different times during the Ca²⁺ imaging experiments. Red arrows indicate internalized C. albicans. b Confocal images showing the localization of InsP₃R in BMDMs incubated with C. albicans. Asterisks indicate C. albicans. c Effect of InsP₃R-modulated Ca²⁺ release on the phagocytosis of BMDM induced by C. albicans. Macrophages were co-cultured with C. albicans for 30 min. Representative live-cell video microscopy still images showing variation in the number of C. albicans engulfed by individual macrophages at 30 min. Arrows indicate the BMDMs that took up C. albicans. The phagocytosis index was calculated as the average number of C. albicans ingested by BMDMs during 30 min. Phagocytosis percentages represent the percentage of BMDMs undergoing C. albicans internalization during 30 min. Data are summarized as the mean ± SEM from three experiments (***p < 0.001)