Fig. 5

SEC5 and TBK1 modulate C. albicans-induced IFN-β production. a Representative immunoblots showing the amount of phosphorylated TBK1 (p-TBK1) in the cytosolic fraction and IRF-3 in the nuclear fraction from RAW264.7 cells at different time points of C. albicans stimulation (MOI = 10). Cells were pretreated with SEC5-shRNA or scramble shRNA (NC-shRNA), and western blots for SEC5 after shRNA treatments are shown for comparison. b Expression of IFN-β in C. albicans-infected RAW264.7 cells pretreated with SEC5-shRNA or scramble shRNA was measured by quantitative real-time PCR at the indicated time points. Data were normalized to the expression of β-actin. c BMDMs were transfected with SEC5-EGFP or EGFP vectors for 48 h, stimulated with C. albicans for 4 h, and then IFN-β production was analyzed by ELISA. d BMDMs were treated with SEC5-siRNA or scramble siRNA for 48 h, stimulated with C. albicans for 4 h, and then IFN-β production was analyzed by ELISA. e BMDMs were treated with 20 nM MRT67307 for 2 h, stimulated with C. albicans for 4 h, and then IFN-β production was analyzed by ELISA. Data are expressed as the mean ± SEM from at least three independent experiments (***p < 0.001)