Fig. 6
SEC5-InsP3R interaction influences TBK1-IRF-3 signaling cascade. a In vitro pull-down of SEC5 and TBK1 in C. albicans-stimulated (MOI = 10) RAW264.7 cell lysates by GST-H1 or GST. Quantification of SEC5 and TBK1 immunoblot signal intensities from GST-H1 pull-down experiments is shown. Data are summarized as the mean ± SEM from three separated experiments. b Confocal images depicting the co-localization of TBK1 and InsP3R on phagosomes (Pearson coefficient = 0.87). White arrow indicates phagosome. c Immunoblots showing TBK1 and p-TBK1 immunoprecipitated using V5 antibodies in the presence of H1-TAT or H1S-TAT recombinant peptides. d Immunoblots depicting the phosphorylation of recombinant IRF-3-C in the presence of H1 peptides. e Immunoblots of in vitro pull-down assays using GST-SEC5-rbd and purified His-SEC5-2. Purified SEC5-rbd inhibited the interaction of SEC5-2 peptides with InsP3R-H1. f Immunoprecipitation examining the interaction of V5-BTK1 and purified His-SEC5-N in the presence of H1-TAT or H1S-TAT recombinant peptides