Fig. 7
AeSSPs are nuclear localized and enhance susceptibility to oomycete infection. a Agrobacterium tumefaciens was used to transiently express GFP on one half of a 3- week-old leaf of N. benthamiana, while AeSSP candidate fused to GFP was expressed on the other half of the leaf. One day after treatment, the infiltrated area was inoculated with Phytophthora capsici zoospore. Photograph (3 dpi) illustrated symptoms observed upon AeSSP1256 expression vs GFP. Black line indicates the agroinfiltrated area. Scale bar = 1 cm. b The average lesion sizes (mm2) were quantified 3 days after P. capsici inoculation. Results presented the means +/− standard error of the mean (SEM) of independent experiments. Asterisk indicates significant differences (Student’s t test, p < 0,05). c A. tumefaciens was used to transiently express either a full-length version of GFP-tagged AeSSPs (+SP, top panels) or a matured form (–SP, lower panels). Photographs are taken 24 hours post inoculation (hpi). Scale bars = 5 μm. d Closer view of the subcellular localization of the full-length version of AeSSP1256 after transient expression in Nicotiana leaf (24 hpi)