Fig. 8

AeSSP1256 is a secreted protein which acts within the plant cell. a Overview of transiently transformed leaf epidermis with A. tumefaciens carrying constructs encoding for a matured (–SP:AeSSP1256:GFP:KDEL) or a full-length (+SP:AeSSP1256:GFP:KDEL) form of AeSSP1256 in fusion with an ER retention signal. Control leaves are transformed with an ER-marker. Confocal imaging was performed 24 hpi. Scale bar = 10 μm. b Nicotiana leaves expressing either the matured form (–SP:AeSSP1256:YFP) or the full-length form of AeSSP1256:YFP (+SP:AeSSP1256:YFP) before (top panel) and after (above panel) treatment with Brefeldin A, a drug that blocks transport of secretory proteins to the Golgi apparatus. Confocal imaging was performed 48 h post agroinfection. Scale bar = 45 μm. c Composite Medicago roots transformed with +SP:AeSSP1256:GFP or a GFP empty vector. Photographs are taken 15 days after A. rhizogenes inoculation