Fig. 6
From: Individual odour signatures that mice learn are shaped by involatile major urinary proteins (MUPs)
Darcin influences the release of male signalling volatiles from urine of laboratory strains. The release of 2-sec-butyl-4,5-dihydrothiazole (SB2HT), (R,R)-3,4-dehydro-exo-brevicomin (DHB), E,E-α-farnesene (αF) and E-β-farnesene (βF) was compared between urine from BALB/c males, B6 males and BALB/c male urine with r-darcin added to equalize that in B6 male urine (1 μg/μl). Volatiles were extracted from 10 μl urine streaked onto filter paper by HS-SPME for 10 min starting 10 min after deposition (n = 7 replicates per urine type) followed by GC-MS analysis. a Peak areas for each volatile according to urine type (boxplots show medians, interquartile and total range). b Principal component analysis derived a single component that accounted for strong correlation in the amount of DHB, βF and αF (76% of variance), with scores differing significantly between urine types (F2,18 = 12.71, p < 0.001). Bonferroni post hoc comparisons assess differences between each urine type (see text)