Fig. 7
From: Individual odour signatures that mice learn are shaped by involatile major urinary proteins (MUPs)
Darcin and MUP7 alter the release pattern of urinary volatiles from B6 male urine. Recombinant MUP (r-darcin, r-MUP7 or r-MUP9, 1 μg/μl) or a buffer control was added to a standard pool of B6 male urine. Volatiles were extracted from 10 μl urine streaked onto filter paper by HS-SPME for 10 min starting 10 min after deposition (n = 10 replicates per urine type) followed by GC-MS analysis. Data are plotted as % change from matched control urine sample (means ± SEM). p values indicate significant differences to control urine (planned contrasts) after MANOVA confirmed a highly significant effect of treatment on the volatiles released (F9,105 = 4.32, p < 0.0001). βF and αF (farnesenes) were summed for analysis, as both were very low in B6 urine (see Fig. 6). Planned contrasts indicated significant differences between urine types for SB2HT (F3,35 = 5.66, p = 0.003) and farnesenes (F3,35 = 6.53, p = 0.001) but not for DHB (F3,35 = 1.03, p = 0.39)