Fig. 5

Validation of changes in IR events and associated alterations in H3K36me2 levels. a, b RNA-seq and ChIP-seq tracks, together with qRT-PCR and ChIP-PCR validations of two representative genes (a and b), which display higher levels of both IR and H3K36me2 at the 5′ end of gene body and lower levels at the 3′ end in 725Ri-1 mutant compared to WT rice plants. Two introns with IRI change validated by qRT-PCR are shown in dashed rectangle. IRI and read numbers (in parentheses) are shown near the corresponding introns. Black arrow pairs denote the positions of six primer sets that were used for ChIP-PCR validation for H3K36me2. Standard deviation (SD) of three qPCR replicates is shown. *, **, and *** stand for p value < 0.05, < 0.01 and < 0.001, respectively (t test)