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Fig. 4 | BMC Biology

Fig. 4

From: Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation

Fig. 4

Selective cell ablation of co-cultures of HeLa cells stably expressing SNR and SNG in mitochondria a Co-cultures were irradiated with ~ 4 W/cm2 blue light for 2 min. Images were taken 0, 3 and 5 h post-irradiation. Only cells expressing SNG underwent cell death; cells expressing SNR survived. b Co-cultures were irradiated with ~ 4 W/cm2 orange light and images were taken as in a. Half of cells expressing SNR underwent cell death during this interval, while all cells expressing SNG survived. c Quantitative analysis for selective cell ablation with 440 nm and 560 nm light irradiation. Under 440 nm light irradiation, significant cell death occurred for cells expressing SNG compared to SNR (p < 0.01, t test, n = 97 cells). For 560 nm light irradiation, significant cell death occurred for cells expressing SNR compared to SNG (p < 0.05, t test, n = 36 cells). Scale bar = 20 μm. Error bar represents ±SEM

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